Firstly, Cas9-mediated genome editing has allowed rapid generation of transgenic model and widens biological research over classic, genetically tractable animal model organisms (Sander, Joung, 2014). CRISPR-based editing could be used to quickly mode the causal roles of particular genetic deviation alternatively of depending on disease models that only phenocopy a specific disorder. By applying this, it could expand novel transgenic animal models ( Wang, et al., 2013) to engineer isogenic embryonic stem cells (ES) and induced pluripotent stem cells (iPS) cell disease models with particular mutations corrected or commenced.(Schwank, et al., 2013). For all these years of cellular models, Cas9 can be effortlessly commenced into the target cells using transient transfection of plasmids carrying Cas9 and suitable designed sgRNA. In addition to that, common diseases—like heart disease, schizophrenia, diabetes and autism— that are usually polygenic can have a promising approach for studying them with the multiplexing capabilities of Cas9.