The tissue of lever mice stock solution: A 0.5 g of liver tissue spiked with Mo nanoparticle (0.25 mg) was prepared by dissolving in aqua regia and gently evaporated several times till dryness and removing any excess of them. The residual was dissolved in distilled water to 10 mL in a measuring flask containing 1mL of concentrated HNO3.
2.3. Recommended procedures
A 0.1 g of OOBAR was mixed with adjust concentration of molybdenum solution, H2SO4, L-ascorbic acid, and NH4SCN then diluted to 25 mL and shaken 60 min at room temperature. The remaining concentrations of Mo(V) were determined using spectrophotometrically (?max 485 nm) 41. The sorption percentage of Mo(V) and sorption capacity of OOBAR (Q, mmol/g) were calculated.
By using a dynamic technique, 10 g of OOBAR was packed through glass column which has 35 cm long and 1.5 cm in diameter with a bed height at L= 15 cm. A series of 25 mL of tap water, liver mice tissue or vitamins solutions (n = 5) were passed through the OOBAR columns at different flow rate 0.2-1.7 mL/min. The effluent solutions were collected and analyzed spectrophotometrically. Mo(V) was eluted from OOBAR columns with NH4OH (0.05 mol/L) as eluent at a flow rate of 3 mL/min then determined spectrophotometrically.